Fig. 1

Study design showing timeline of disease induction, immunostaining, PET/CT imaging, and ex vivo analyses. Twenty female C57BL/6 J mice were induced with EAE via subcutaneous administration of myelin oligodendrocyte glycoprotein (MOG_1–125) and intraperitoneal injection of pertussis toxin (PTX). Mice were subsequently monitored for signs of paralysis from day 8 onwards. On day 14, n = 6 mice with hindlimb paralysis (scores 2.5–3.5) and n = 5 naive mice were intravenously injected with [⁶⁴Cu]CD19-mAb and imaged using PET/CT 24 h later. The remaining EAE mice were used for in vitro autoradiography/blocking and immunostaining studies