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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Oxidized cell-free DNA as a stress-signaling factor activating the chronic inflammatory process in patients with autism spectrum disorders

Fig. 3

Total NF-κB (p65) expression and nuclear translocation of p65 as parameters of response to DNAoxy. PBL were cultured with DNAoxy (50 ng/ml) for various time durations up to 24 h. a Total NF-κB (p65) expression was quantified by flow cytometry. Expression level was measured as fold change of intensity relative to untreated zero time-point set in the control group (horizontal green line, 1.0 ± 0.3 arb.un.). For healthy controls, no change in expression level of p65 had been observed; for ASD patients, significant increase in p65 expression was found both at 0 h and 24 h time-points. Data are shown as means ± SD. b Relative cell number with intranuclear p65 location. PBL number with intranuclear p65 location were assessed by Cytell Cell Imaging System and expressed relative to untreated zero time-point set in the control group (horizontal green line, 1.0 ± 0.3 arb.un.). Data are shown as means ± SD. c Reads of the CyTell cell imaging system (GE Healthcare). The red sectors indicate the fraction of NFκB-positive nuclei, while the blue sectors indicate the fractions of NFκB-negative cell nuclei. The time of cultivation with DNAoxy (50 ng/ml) are indicated in the figure. Control, PBL of healthy controls; group 1, PBL of patients with mild-to-moderate ASD. *p value < 0.01 compared to untreated zero time-point set in the control group (Mann–Whitney U test

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