Fig. 2

The protein levels of CX3CL1 and CX3CR1 in the brain tissues of patients and rats after SAH. a Western-blot analysis of protein levels of CX3CL1 and CX3CR1 in brain tissues of patients. b Western-blot analysis showed the protein levels of CX3CL1 and CX3CR1 at 3 h, 6 h, 12 h, 24 h, 72 h and 7 days after SAH. c, d Quantification of protein levels of CX3CL1 and CX3CR1 at different time points. e Double-immunofluorescence analysis was performed with CX3CL1 (green) and a neuronal marker (NeuN, red) in sections. Nuclei were fluorescently labeled with 4′-6-diamidino-2-phenylindole (DAPI) (blue). Arrows point to CX3CL1-positive neurons. Scale bar = 20 μm. f Double-immunofluorescence analysis was performed with CX3CR1 (green) and a microglia marker (Iba-1, red) in sections. Nuclei were fluorescently labeled with DAPI (blue). Scale bar = 20 μm. In (c, d), the mean values for the Sham group were normalized to 1.0, and data are mean ± standard deviations (SD), n = 6. *P < 0.05, **P < 0.01 vs sham group