Fig. 7

The effect of CX3CL1/CX3CR1 overexpression on the delivery of exosomal microRNA-124 (miR-124). a Triple-immunofluorescence analysis was performed with cy3-miR-124 (red), a neuronal marker (NeuN, blue), and a microglia marker (Iba-1, purple) in co-cultured neurons and microglia. Scale bar = 20 μm. b Immunofluorescence analysis was performed with neuron-specific enolase (NSE) antibodies (green) in co-cultured neurons and microglia observed by stimulated emission depletion microscopy. c Scanning electron micrograph of exosomes isolated from medium. d Real-time quantitative polymerase chain reaction (RT-qPCR) assay of the exosomal miR-124 levels in medium. In d, the mean values for the control group were normalized to 1.0, and all data are mean ± SD, n = 3. ***P < 0.001 vs control + vehicle group; ^###P < 0.001vs OxyHb + vehicle group; ^$$$P < 0.001 vs control group