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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Inhibition of leucine-rich repeats and calponin homology domain containing 1 accelerates microglia-mediated neuroinflammation in a rat traumatic spinal cord injury model

Fig. 4

LRCH1 knockdown increases microglia-mediated N27 cell death. a Representative dot plots showing the apoptosis and necrosis of N27 cells after co-culture with primed lentivirus-infected microglia in Transwell plates. LC, N27 cells cultured with microglia infected with control lentivirus. LL, N27 cells cultured with microglia infected with LRCH1 shRNA-encoding lentivirus. Un, unprimed microglia. LPS+ATP, primed microglia. b Statistics for the frequencies of Annexin V+ cells and PI+ cells. Note that PI+ cells include PI+Annexin V+ cells and PI+Annexin V- cells. n = 6 in 3 independent experiments. ns, non-significant. c Viability of primary rat spinal cord neurons after co-culture with lentivirus-infected microglia for 24 h. n = 6 in 2 independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001

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Journal of Neuroinflammation

ISSN: 1742-2094