Fig. 9

FoxO1 participates in H2R/H3R-mediated microglia TLR4 expression and inflammatory factor release. a The protein levels of FoxO1 were detected by Western blotting using specific antibody in primary microglia. b, d The protein levels of TLR4 were detected by Western blotting using specific antibody in primary microglia, and expression of TLR4 was quantified and normalized to β-actin levels. Each value was expressed relative to that in the control group, which was set to 100. c, e Concentration of TNF-α and IL-1β measured by ELISA. **P < 0.01 versus the control group. ^##P < 0.01 versus the LPS group. ^{^}P < 0.05 versus the LPS + Amth group. ^{^}P < 0.05 and ^{^^}P < 0.01 versus the LPS + Meth group (n = 3). The data are presented as the mean ± SEM