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Table 1 Groups and number of mice used for each experiment

From: BDNF reverses aging-related microglial activation

Experiment No. of groups No. of animals
Age effects on microglial activation in region of interest (Fig. S1) 2 (3 and 12 months) 6 (3/group)
Expression of Iba-1, p-p65/p65, BDNF, TrkB, TNF, IL-6, TH, and DAT in the SN of mice (Figs. 1a, c, e–i, 2e–h, and 7a–b) 3 (3, 6, and 12 months) 24 (8/group)
Flow cytometry assay of number of Iba1+ cells in the brain of mice (Fig. 1b) 3 (3, 6, and 12 months) 9 (3/group)
Expression of MHC class II in the SN of mice (Fig. 1d) 3 (3, 6, and 12 months) 15 (5/group)
Dual immunofluorescence staining of BDNF/TrkB and Iba1 in the SN of mice (Fig. 2a–d) 2 (6 and 12 months) 6 (3/group)
Effects of subcutaneous BDNF supplementation on expression of Iba1in the SN of age mice (Fig. 3a) 3 (0, 0.1, and 1 μg of BDNF) 14 (4, 5, and 5)
Effects of intra-brain infusion of BDNF on systemic LPS-induced local microglial activation in mice (Fig. 3b and S2a) 3 (SN, striatum, and hippocampus)
Each mouse received saline injection to the left hemisphere and BDNF to the right hemisphere.
12 (4/group)
Effects of knockdown of TrkB on the microglial activation in the SN, striatum, and hippocampus of mice (Figs. 3c and S2b) 3 (SN, striatum, and hippocampus)
Each mouse received shLacZ virus injection to the left hemisphere and shTrkB viruses to the right hemisphere.
9 (3/group)
Primary microglial cultures (Figs. 4e–h and 7d) not applicable 42 pups