Fig. 1

Immunohistochemical analysis of lymphoid cells in the brains of ⍺-synuclein transgenic mice. a Representative bright field light microscopy images from the neocortex, hippocampus, striatum, and thalamus of non-tg and ⍺-syn tg mice immunostained with an antibody against CD3 (general T cell marker). b Computer-based image analysis showing significant increase of CD3 positive cell numbers in neocortex, hippocampus, and striatum of ⍺-syn tg mice. c Representative bright field light microscopy images from the neocortex, hippocampus, striatum, and thalamus of non-tg and ⍺-syn tg mice immunostained with an antibody against CD20 (general B cell marker). d Computer-based image analysis showing comparable numbers of CD20 positive cell in the brains of non-tg and ⍺-syn tg mice. e Representative bright field light microscopy images from the neocortex, hippocampus, striatum, and thalamus of non-tg and ⍺-syn tg mice immunostained with an antibody against CD4 (helper T cell marker). f Computer-based image analysis showing significant increase of CD4 positive cell numbers in neocortex, hippocampus and striatum of ⍺-syn tg mice. g Representative bright field light microscopy images from the neocortex, hippocampus, striatum, and thalamus of non-tg and ⍺-syn tg mice immunostained with an antibody against CD8 (cytotoxic T cell marker). h Computer-based image analysis showing significant increase of CD8 positive cell numbers in neocortex, hippocampus, and striatum of ⍺-syn tg mice. Scale bars = 40 μm (low magnification) and 10 μm (high magnification). Non-tg and α-syn tg mice (n = 4). Statistical significance determined by unpaired t test; *p ≤ 0.05