Fig. 5
Immunohistochemical analysis of microglial cells and astrocytes in the brains of ⍺-synuclein transgenic mice. a Representative bright field light microscopy images from the neocortex, hippocampus, striatum, and thalamus of non-tg and ⍺-syn tg mice immunostained with an antibody against Iba1 (microglial cell marker). b Computer-based image analysis showing increased numbers of Iba1 positive cell in the neocortex, hippocampus, and striatum of non-tg and ⍺-syn tg mice. c Representative bright field light microscopy images from the neocortex, hippocampus, striatum, and thalamus of non-tg and ⍺-syn tg mice immunostained with an antibody against GFAP (astroglial cell marker). d Computer-based image analysis showing increased numbers of GFAP positive cells in the neocortex, hippocampus, and striatum of non-tg and ⍺-syn tg mice. Scale bars = 40 μm (low magnification) and 10 μm (high magnification). Non-tg and α-syn tg mice (n = 4). Statistical significance determined by unpaired t test; *p ≤ 0.05