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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Three-dimensional morphometric analysis reveals time-dependent structural changes in microglia and astrocytes in the central amygdala and hypothalamic paraventricular nucleus of heart failure rats

Fig. 3

HF-induced deramification and increased the proportion of pro-inflammatory microglia in the PVN and CeA. a Scheme depicts the HF-induced transition from ramified to deramified microglia. Red circles depict superimposed spheres centered around microglia somata used for Sholl analysis. b, c Bar graphs show the mean number of total of Sholl intersections for PVN and CeA microglia in sham rats (n = 12, pooled) and HF rats at 8, 14, and 16 weeks post-surgery (n = 4/group). d, e Bar graphs show the mean proportion of deramified microglia in the PVN and CeA in sham rats (n = 12, pooled) and HF rats at 8, 14, and 16 weeks post-surgery (n = 4/group). f, g Mean distribution plots of the number of Sholl intersections as a function of the distance from the microglial soma for sham and HF rats 16 weeks post-surgery (n = 4/group). h Distribution plot comparing Sholl intersections as a function of the distance from the microglial soma for PVN and CeA microglia (n = 12/group, pooled). *p < 0.05, **p < 0.01, and ***p < 0.0001 vs. respective sham, two-way ANOVA, or one-way ANOVA followed by Tukey’s post hoc test

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