Fig. 1

Immunolabeling of versican V0/V1 in wild-type and EXTL2^−/− lesions. a Representative images of tissue sections stained with the anti-versican V0/V1 antibody (left) and the secondary antibody control. Scale bar = 200 μm. b Representative lesion from wild-type day 14 animal stained with eriochrome cyanine R (scale bar = 100 μm) and c immunofluorescence staining for versican V0/V1, MBP, and merged image. Scale bar on immunofluorescent image = 200 μm. Inset shows high magnification image of versican and MBP. d Representative images of versican V0/V1 in wild-type and EXTL2^−/− lesions at 14 and 21 dpl. White outline indicates approximate lesion area. Scale bar = 200 μm. Quantification of versican area within the lesions, in terms of percent area of the whole lesion determined separately by MBP staining, and in terms of absolute area within the isolated lesion are shown in e for day 14 and f for day 21. Graphs show mean with standard deviation. Two-tailed, unpaired Student’s t test. *p < 0.05, **p < 0.01