Fig. 2

Immunostaining and quantification of oligodendrocyte lineage cells following injury. Representative images of lesions labeled with Olig2, PDGFRα, and APC (CC1) are shown in a and e, for days 14 and 21, respectively. Scale bar = 200 μm. Insets are high magnification images of individual Olig2⁺ nuclei co-labeled with PDGFRα or APC. Density of all Olig2⁺ nuclei are quantified for day 14 in b and day 21 in f. Proportion of Olig2⁺PDGFRα⁺ OPCs at both day 14 and 21, calculated as a fraction of all Olig2⁺ cells, are shown in c and g; c shows day 14 and g shows day 21 analyses. Graphs show mean with standard deviation. Two-tailed, unpaired Student’s t test