Fig. 3

Assessment of area of lesion by loss of MBP immunoreactivity and changes in axonal density in wild-type vs EXTL2^−/− lesions at day 14. a Representative images of MBP immunofluorescent staining in wild-type and EXTL2^−/− mice. Scale bar = 200 μm. Lesion area determined by loss of MBP immunoreactivity was quantified for both day 14 (b) and day 21 (c). Two-tailed, unpaired Student’s t test. d Representative images from day 14 lesions labeled with MBP and NF200 to denote demyelinated area and axons, respectively. Scale bar = 200 μm. e Total number of axons per lesion. f Density of axons calculated by dividing number of axons over lesion area. Graphs show mean with standard deviation. Two-tailed, unpaired Student’s t test. *p < 0.05