Fig. 7

Effect of BMDM conditioned media on cultured neurons and OPCs. a Fold change in number of MAP2+ cells assessed for cultured neurons 24 h following application of CSPG-stimulated BMDM conditioned media (CM) (data pooled from 3 independent experiments of quadruplicate cultures each). Unpaired, two-way Student’s t test. b Images of neurons labeled with MAP2 and nuclear yellow; scale bar = 100 μm. c Representative images of wild-type (WT) OPCs labeled with O4 and nuclear yellow 24 h following treatment with BMDM conditioned media. Scale bar = 200 μm. d Comparison of wild-type and EXTL2^−/− BMDM conditioned media on OPC outgrowth. Fold change represents data pooled from 3 independent experiments. Two-way ANOVA with Tukey’s multiple comparisons test. n.s. = not significant. *p < 0.05. e Representative images of MBP⁺ oligodendrocytes 72 h following seeding into a 96-well plate with BMDM conditioned media. Scale bar = 100 μm. f Comparison of wild-type and EXTL2^−/− BMDM conditioned media on OPC differentiation 72 h after seeding. Two-way ANOVA with Sidak’s multiple comparisons test. n.s. = not significant. g Comparison of number of OPCs seeded either in the standard OPC media (as described in the methods section) or minimal media (MM) of DMEM containing only GlutaMAX™, sodium pyruvate, and penicillin/streptomycin. h Representative images of O4-stained OPCs 24 h post-seeding in either standard OPC media or MM. Scale bar = 100 μm. i Comparison of OPC process outgrowth 24 h post-seeding in MM, treated with conditioned media from unstimulated or CSPG-stimulated BMDMs. All graphs are mean with SEM. Two-way ANOVA with Sidak’s multiple comparisons test. n.s. = not significant