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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: A1 reactive astrocytes and a loss of TREM2 are associated with an early stage of pathology in a mouse model of cerebral amyloid angiopathy

Fig. 5

Cholesterol accumulation is associated to vascular amyloid in Tg-FDD mice. a Double immunofluorescence of smooth muscle actin (SMA, red) and cholesterol fFilipin, green) in Tg-FDD mice show filipin+ staining without association to SMA (Merge). Colocalization analysis (CC) was performed to determine pixel intensity correlation between filipin and SMA. White pixels indicate colocalization between filipin and SMA signal. Plot profiles of representative intensities showing almost no overlapping intensities of cholesterol (filipin, green) and SMA (red). b Double immunofluorescence of Danish amyloid (ADan, red) and cholesterol (filipin, green) in Tg-FDD mice show strong filipin+ colocalization and overlapping intensities (Merge). Colocalization analysis confirmed major association between filipin and vascular amyloid. White pixels indicate colocalization between filipin and ADan (red) signal. Plot profiles of representative intensities showing major overlapping intensities of cholesterol (filipin, green) and ADan (red). c Double immunofluorescence of astrocytes (GFAP, red) and cholesterol (filipin, green) in Tg-FDD mice show filipin+ show a high degree of cellular association (Merge). White pixels in CC indicate colocalization between filipin and GFAP (red) signal. Plot profiles of representative intensities showing minor overlapping intensities of cholesterol (filipin, green) and GFAP (red). Scale bar 25 μm

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