Skip to main content
Fig. 8 | Journal of Neuroinflammation

Fig. 8

From: A1 reactive astrocytes and a loss of TREM2 are associated with an early stage of pathology in a mouse model of cerebral amyloid angiopathy

Fig. 8

A1 astrocyte conditioned media decreases TREM2 protein in mouse primary microglia culture. a Schematic diagram showing A1 astrocytes induction by cytokine cocktail treatment (TNFα, IL-1α, and C1q) followed by microglia incubation with astrocyte conditioned media (ACM) from A1 astrocytes or control astrocytes solely treated with PBS. b Double immunofluorescence images of microglia (IBA1, red) and TREM2 (green) of primary WT mouse microglia culture treated with control or A1 ACM. Right panel shows orthogonal images of reconstructed three-dimensional views. c Intensity quantification of TREM2 per cell shows a decrease in TREM2 proteins in microglia incubated with A1 ACM. d Quantification of IBA1+ cell surface area shows no change in cell surface area between control and A1 ACM-treated groups. For quantifications, error bars represented ± SEM where eight to ten images were used for each experiment with n = 3 independent cultures and 20 cells per culture, where ****p < 0.0001 by unpaired Student’s t test. Scale bar 50 μm

Back to article page