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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: NF-κB p65-dependent transcriptional regulation of histone deacetylase 2 contributes to the chronic constriction injury-induced neuropathic pain via the microRNA-183/TXNIP/NLRP3 axis

Fig. 4

miR-183 relieves neuropathic pain in CCI rats via inhibition of TXNIP. a Binding sites between miR-183 and TXNIP predicted by the microrna.org website. b Binding relationship between miR-183 and TXNIP detected by dual-luciferase reporter assay (n = 3). c miR-183 expression and TXNIP mRNA and protein expression in spinal cord of CCI rats determined using RT-qPCR and Western blot analysis, normalized to U6 and GAPDH, respectively (n = 10). d Expression of TXNIP in Iba-1-labeled microglial cells of CCI rat spinal cord detected by immunofluorescence staining (scale bar = 50 μm) (n = 5). e miR-183 expression in microglial cells treated with miR-183 mimic determined by RT-qPCR, normalized to U6 (n = 3). f TXNIP expression in microglial cells treated with miR-183 mimic determined using RT-qPCR and Western blot analysis, normalized to GAPDH (n = 3). g, h PWT and PWL of CCI rats injected with lentivirus expressing miR-183 agomir or in combination with oe-TXNIP following 14 days (n = 10). i miR-183 expression in spinal cord of CCI rats injected with lentivirus expressing miR-183 agomir or in combination with oe-TXNIP determined by RT-qPCR, normalized to U6 (n = 10). j TXNIP expression in spinal cord of CCI rats injected with lentivirus expressing miR-183 agomir or in combination with oe-TXNIP determined using RT-qPCR and Western blot analysis, normalized to GAPDH (n = 10). *relative to microglial cells treated with mimic NC or sham-operated rats,, #relative to CCI rats, and $relative to CCI rats injected with agomir NC indicate p < 0.05. All measurement data were expressed as the mean ± standard deviation. Unpaired t test was used to analyze data between the two groups, one-way ANOVA and Tukey’s post hoc test to analyze data among multiple groups, and repeated-measures ANOVA and Bonferroni post hoc test to analyze data at different time points

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