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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: NF-κB p65-dependent transcriptional regulation of histone deacetylase 2 contributes to the chronic constriction injury-induced neuropathic pain via the microRNA-183/TXNIP/NLRP3 axis

Fig. 6

NF-κB p65 facilitated neuropathic pain in CCI rats by affecting the miR-183-TXNIP-NLRP3 axis via HDAC2. CCI rats were injected with lentivirus expressing PDTC or PDTC + oe-HDAC2. a miR-183 expression in the spinal cord of CCI rats determined by RT-qPCR, normalized to U6 (n = 10). b Expression of TXNIP, NLRP3, ASC, and caspase-1 in spinal cord of CCI rats measured using Western blot analysis, normalized to GAPDH. c IL-1β expression in spinal cord of CCI rats measured using ELISA (n = 10). d Molecular level and neuropathic pain-related behavioral changes in rats following 14 days of injection. e, f HDAC2 expression in spinal cord of CCI rats determined using RT-qPCR and Western blot analysis, normalized to GAPDH (n = 10). g PWT and PWL of CCI rats were measured. *relative to sham-operated rats, #relative to CCI rats, and $relative to CCI rats injected with PDTC indicate p < 0.05. All measurement data were expressed as the mean ± standard deviation. Unpaired t test was used to analyze data between the two groups, one-way ANOVA and Tukey’s post hoc test to analyze data among multiple groups, and repeated-measures ANOVA and Bonferroni post hoc test to analyze data at different time points

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Journal of Neuroinflammation

ISSN: 1742-2094