Fig. 1

Preventive treatment with compound 1 results in delayed EAE onset. a One experiment, representative of two independent experiments, is presented. Each experimental group included 11 mice. In the preventive protocol, 1 was administered intraperitoneally (30 mg/kg, once/day) at 3 dpi (solid arrow); in the therapeutic protocol, 1 was administered at disease onset (10 dpi, dashed arrow). Daily clinical scores are shown as mean ± SEM. b, c Area under the curve (AUC) of EAE clinical course in mice treated, or not, with 1 (as in panel a) was calculated considering the period from the disease onset to 14 dpi or during the overall EAE course (from onset to 28 dpi). Data are shown as mean ± SEM. d EAE course in mice treated with 1 (30 mg/kg, twice/day, 11 mice/group) as preventive protocol from the day of immunization. Daily clinical scores are shown as mean ± SEM. e, f AUC of EAE clinical score calculated considering the whole EAE course (e) and Kaplan-Meier analysis of disease onset (f) in mice treated, or not, with 1 (as in panel d). g EAE course in mice treated with 1 (30 mg/kg, twice/day, 11 mice/group) as therapeutic protocol, starting at disease onset. Daily clinical scores are shown as mean ± SEM. h, i AUC of EAE clinical course in mice treated, or not, with 1 (as in panel g) was calculated considering the period from the disease onset to 15 dpi (h) or during the overall EAE course (from onset to 28 dpi, panel i). Data are shown as mean ± SEM. *p < 0.05; **p < 0.01; ****p < 0.0001. Data were analyzed by t test, except for data in panel f (analyzed using the Gehan-Breslow-Wilcoxon test)