Fig. 3

Sirt6 inhibition determines a delayed influx of CXCR4⁺ DC in lymph nodes of EAE-affected mice. Mice were treated, or not, following the preventive protocol (i.p. administration of 30 mg/kg 1, twice/day). a One representative plot of CD11c⁺ DC stained CD40, CCR7, and CXCR4 is shown. b–d DC were obtained from lymph nodes (LNs) at 7 dpi and stained with antibodies against CD11c (b), or against CD11c and CD40 (c), or against CD11c and CXCR4 and CCR7 (d) and analyzed by FACS. Data are shown as mean ± SEM. **p < 0.01 (n = 6 from two independent experiments). e–g DC were obtained from LNs at 10–13 dpi, i.e., at disease onset in vehicle-treated mice. Cells were stained with antibodies against CD11c (e) or against CD11c and CD86 (f), or against CD11c and CXCR4 and CCR7 (g). Data are shown as mean ± SEM. *p < 0.05, **p < 0.01 (n = 6 from two independent experiments). Data were analyzed by t test