Fig. 4

Effect of Sirt6 inhibition on chemotaxis in DC. a BMDDC were isolated from wild-type mice, incubated for 18 h in the presence or absence of 50 μM compound 1 and homogenized; Western blot analyses were then performed to evaluate the level of acetylated H3K9 (H3K9-Ac). A representative Western blot analysis is shown, together with the normalized quantification of the band intensity (n = 3). b, c DC were isolated and stimulated (or not) with LPS, in the presence or absence of 50 μM 1 for 18 h. DC were then challenged to migrate toward a medium containing, or not, 500 ng/ml CCL19/CCL21 (b) or 300 ng/ml SDF-1 (c). Migration was measured in ChemoTx chambers, and the chemotaxis index (CI = number of cells that migrated toward chemoattractant/number of cells that migrated toward medium) was calculated. Results shown are the mean ± SD of three experiments. *p < 0.05; **p < 0.01, compared to the corresponding control. Data were analyzed by t test