Fig. 5

Effect of compound 1 on IFNγ, IL12, and IL10 profile in EAE. Mice were treated (or not) with 1 (administered twice/day), following the preventive protocol (see the “Materials and methods” section). a At 7 dpi and at 13–17 dpi (i.e., onset for vehicle-treated mice), mice were sacrificed and TNFα was evaluated in the collected plasma. b, c Spinal cords were collected at 13–17 dpi (i.e., onset for vehicle-treated mice), and the relative expression of IFNγ, IL12, and IL10 (b) and of TBP, HPRT1, and β-actin (used as “housekeeping” genes) (c) was analyzed by qPCR analysis. Samples from at least 4 animals were analyzed for each condition. *p < 0.05, compared to controls (data analyzed by t test)