Skip to main content
Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Chitotriosidase, a biomarker of amyotrophic lateral sclerosis, accentuates neurodegeneration in spinal motor neurons through neuroinflammation

Fig. 6

Effect of CHIT-1 on motor neurons and axonal density. Representative confocal images (merged) of spinal cord sections co-labeled for ChAT (green) and SMI-31 (red) in control [buffer, (a)], CHIT-1 [C50, 100, 200, 500 pg; (be)] and also ALS-CSF injected (f) groups. Scale bar = 150 μm (for all images). CHIT-1 induces loss of ChAT positive motor neuronal cells, similar to ALS-CSF, at a dose of 500 pg. (**p < 0.01 C500 vs. NC; ***p < 0.001 ALS vs. NC; $$p < 0.01 C500 and ALS vs. buffer) (g). Histogram representing area of phosphorylated neurofilaments (SMI-31) in white matter of ventral horn of spinal cord (h). Representative images of Nissl-stained spinal cord sections of normal control [NC, (i)], Buffer (j), CHIT-1 [C50, C100, C200, C500 pg; (kn)] and ALS-CSF (o) injected groups. CHIT-1 (500 pg) and ALS-CSF results in motor neuronal loss (*p < 0.05 C500 vs NC; ***p < 0.001 ALS vs. NC; $$p < 0.01 C500 vs buffer; $$$p < 0.001 ALS vs. buffer) (p)

Back to article page