Fig. 2

Memantine decreases AQP4 and GFAP loss in IgG(AQP4+) mice. Pictures are representative photomicrographs of cross sections showing the white matter in ventrolateral region of cervical spinal cord. a Double immunofluorescence staining of AQP4 and GFAP (astrocyte marker) in IgG(AQP4+) mice treated with preventive memantine or vehicle at 1, 4, and 8 dpi or with therapeutic memantine or vehicle at 8 dpi. IgG(con) mice treated with vehicle were used as a sham control. Sections were counterstained with DAPI. Scale bar = 100 μm. b Relative intensity of AQP4 immunofluorescence in the spinal cord. c Relative intensity of GFAP immunofluorescence in the spinal cord. n = 5 per group. Data are mean ± SEM. One-way ANOVA with Tukey-Kramer post hoc test. ns, not significant; *p < 0.05; ***p < 0.001