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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Microglial and peripheral immune priming is partially sexually dimorphic in adolescent mouse offspring exposed to maternal high-fat diet

Fig. 3

mHFD effect on plasma cytokine profile and brain inflammatory profile of PND30 offspring 8 h after LPS treatment. a IL-1β, b IL-17, c TNF-α, d IL-6, and e IL-10 were measured by multiplex-ELISA in offspring plasma after administration of SAL or LPS by intraperitoneal injection. Dotted line on the graphs indicates the detection limits. f Radar graph illustrates cytokines levels in offspring from both diet groups regardless of their sex 8 h after LPS immune challenge. g Nfκb and h Cox2 mRNA levels were evaluated by rt-qPCR in offspring hippocampus after administration of SAL or LPS by intraperitoneal injection. Data are shown as mean ± standard error of the mean. p < 0.05*, p < 0.001***, p < 0.0001**** (treatment) by mixed-effects analysis, p < 0.05# (treatment × diet), p < 0.01# (treatment × sex × diet), p < 0.001 # # # (treatment × sex or treatment × sex × diet) by mixed-effects analysis. CD, control; Cox2, Cyclooxygenase 2; LPS, lipopolysaccharide; IL, interleukin; SAL, saline; mHFD, maternal high-fat diet; Nfκb, nuclear factor kappa B; TNF-α, tumor necrosis factor α

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