Fig. 4

mHFD effect on microglia-related mRNA expression in the hippocampus of PND30 offspring. a Scheme illustrating the region of interest, the hippocampus, in sagittal and coronal views of a mouse brain. mRNA expression (2^-ΔΔCt­) was evaluated for Tgfb1 (b), Aif1 (c), Tmem119 (d), Trem2 (e), and Cx3cr1 (f) normalized by housekeeping gene Rpl32. Data are shown as 2^(-ΔΔCt­ _Mean ± standard error of the mean). p < 0.001**, p < 0.001***, p < 0.0001**** (sex × diet) by 2-way ANOVA followed by Bonferroni post-hoc test. Aif1, allograft inflammatory factor 1; a.u., arbitrary units; CD, control; Ct, cycle threshold; Cx3cr1, fractalkine receptor; mHFD, maternal high-fat diet; Tgfb1, transforming growth factor β1; Tmem119, transmembrane protein 119; Trem2, triggering receptor expressed by myeloid cells 2