Fig. 7
mHFD effect on dark perivascular cells, dark microglial cells, and apoptotic cells in dorsal hippocampus CA1 of PND30 offspring. a Dark perivascular cells, b dark microglia, and c apoptotic cells examples are presented. Dark perivascular cell density was counted in the d st rad and the e st lac mol. Ultrastructural analysis of dark cells in the st lac mol revealed differences in their f–j dilation of the endoplasmic reticulum and Golgi apparatus cisternae. Dark processes are pseudo-colored in red. Presynaptic and postsynaptic elements are pseudo-colored respectively in green and orange. Mitochondria are pseudo-colored in purple. Blood vessels are identified by “BV.” Dark perivascular cell, dark microglia, and apoptotic cell are respectively identified using “DP,” “DM,” and “AC.” Dilated endoplasmic reticulum and Golgi apparatus are identified by a white asterisk. Scale bar for representative picture of cell type is equivalent to 5 μm while scale bar for picture showing endoplasmic reticulum and Golgi apparatus is equivalent to 500 nm. Data are shown as mean ± standard error of the mean. p < 0.05* (sex) by 2-way ANOVA, p < 0.05# (sex × diet) by 2-way ANOVA followed by Bonferroni post-hoc test. ♀, female; ♂, male; CD, control; Dilated ER/Golgi, dilated endoplasmic reticulum and Golgi apparatus cisternae; mHFD, maternal high-fat diet