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Table 1 Primers sequence for rt-qPCR experiments

From: Microglial and peripheral immune priming is partially sexually dimorphic in adolescent mouse offspring exposed to maternal high-fat diet

Targets

Primer 5′

Primer 3′

Nfκb

CAC CTA GCT GCC AAA GAA GG

GCA GGC TAT TGC TCA TCA CA

Cox2

TGF TGG TGG AAA AAC CTC GT

GGT GCT CGG CTT CCA GTA TT

Tmem119

TTC TTC CGG CAG TAC GTG AT

CGA GGA TGG GTA GTA GGC TG

Aif1

TCT GCC GTC CAA ACT TGA AG

GCC ACT GGA CAC CTC TCT AA

Trem2

ACC CTC TAG ATG ACC AAG ATG C

TTG GGC ACC CTC GAA ACT C

Cx3cr1

CAA GCT CAC GAC TGC CTT CT

TGT CCG GTT GTT CAT GGA GTT

Tgfb1

ACA TGT GGA ACT CTA CCA GAA A

CTG CCG TAC AAC TCC AGT GA

Rpl32

TTG TTG CTC CCA TAA CCG ATG

TTA AGC GAA ACT GGC GGA AAC

Gapdh

GGA GAA ACC TGC CAA GTA TGA

GGT CCT CAG TGT AGC CCA AG

  1. Aif1 allograft inflammatory factor 1, Cox2 cyclooxygenase 2, Cx3cr1 fractalkine receptor, Gapdh glyceraldehyde 3-phosphate dehydrogenase, Nfκb nuclear factor kappa B, Tgfb1 transforming growth factor β1, Tmem119 transmembrane protein 119, Trem2 triggering receptor expressed by myeloid cells 2
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Journal of Neuroinflammation

ISSN: 1742-2094