Gene | Treatment | Male | Female | F | p | ||
---|---|---|---|---|---|---|---|
CD | mHFD | CD | mHFD | ||||
Nfκb | SAL | 2−ΔΔCt = 1.000 ΔΔCt = 0.000 ±0.344 | 2−ΔΔCt = 0.090 ΔΔCt = 2.633 ± 0.833 | 2−ΔΔCt = 1.030 ΔΔCt = 0.038 ± 0.360 | 2−ΔΔCt = 1.040 ΔΔCt = 0.630 ± 0.569 | Treatment × sex × diet, 5.344 Treatment × sex, 5.513 Treatment × diet, 2.523 Sex × diet, 8.428 Treatment, 5.646 Sex, 8.928 Diet, 2.926 | Treatment × sex × diet, 0.0265* Treatment × sex, 0.0243* Treatment × diet, 0.1207 Sex × diet, 0.0062** Treatment, 0.0228* Sex, 0.0050** Diet, 0.0955 |
LPS | 2−ΔΔCt = 1.190 ΔΔCt = 0.255 ± 0.385 | 2−ΔΔCt = 1.020 ΔΔCt = 0.034 ± 0.317 | 2−ΔΔCt = 1.210 ΔΔCt = 0.270 ± 0.281 | 2−ΔΔCt = 1.330 ΔΔCt = 0.415 ± 0.175 | |||
Cox2 | SAL | 2−ΔΔCt = 1.000 ΔΔCt = 0.000 ± 0.182 | 2−ΔΔCt = 0.340 ΔΔCt = 1.574 ± 0.312 | 2−ΔΔCt = 1.070 ΔΔCt = 0.101 ± 0.324 | 2−ΔΔCt = 1.100 ΔΔCt = 0.133 ± 0.242 | Treatment × sex × diet, 3.711 Treatment × sex, 2.052 Treatment × diet, 5.596 Sex × diet, 3.170 Treatment, 7.608 Sex, 7.507 Diet, 1.419 | Treatment × sex × diet, 0.0618 Treatment × sex, 0.1604 Treatment × diet, 0.0233* Sex × diet, 0.0832 Treatment, 0.0090** Sex, 0.0094** Diet, 0.2412 |
LPS | 2−ΔΔCt = 0.980 ΔΔCt = 0.022 ± 0.490 | 2−ΔΔCt = 1.200 ΔΔCt = 0.264 ± 0.342 | 2−ΔΔCt = 1.230 ΔΔCt = 0.293 ± 0.289 | 2−ΔΔCt = 1.430 ΔΔCt = 0.516 ± 0.242 | |||
Tgfb1 | – | 2−ΔΔCt = 1.000 ΔΔCt = 0.000 ± 0.218 | 2−ΔΔCt = 0.2764 ΔΔCt = 1.860 ± 0.262 | 2−ΔΔCt = 1.139 ΔΔCt = − 0.190 ± 0.407 | 2−ΔΔCt = 1.282 ΔΔCt = − 0.360 ± 0.335 | Sex × diet, 10.05 Sex, 14.16 Diet, 6.965 | Sex × diet, 0.0050** Sex, 0.0013** Diet, 0.0162* |
Aif1 | – | 2−ΔΔCt = 1.000 ΔΔCt = 0.000 ± 0.328 | 2−ΔΔCt = 0.7169 ΔΔCt = 0.480 ± 0.340 | 2−ΔΔCt = 1.069 ΔΔCt = − 0.100 ± 0.290 | 2−ΔΔCt = 1.196 ΔΔCt = − 0.260 ± 0.299 | Sex × diet, 1.035 Sex, 1.783 Diet, 0.2588 | Sex × diet, 0.3218 Sex, 0.1976 Diet, 0.6168 |
Tmem119 | – | 2−ΔΔCt = 1.000 ΔΔCt = 0.000 ± 0.313 | 2−ΔΔCt = 0.1346 ΔΔCt = 2.89 ± 0.269 | 2−ΔΔCt = 1.260 ΔΔCt = − 0.330 ± 0.350 | 2−ΔΔCt = 1.101 ΔΔCt = − 0.140 ± 0.265 | Sex × diet, 19.47 Sex, 30.15 Diet, 25.34 | Sex × diet, 0.0003*** Sex, < 0.0001**** Diet, < 0.0001**** |
Trem2 | – | 2−ΔΔCt = 1.000 ΔΔCt = 0.000 ± 0.282 | 2−ΔΔCt = 0.1690 ΔΔCt = 2.570 ± 0.229 | 2−ΔΔCt = 0.9781 ΔΔCt = 0.030 ± 0.326 | 2−ΔΔCt = 0.9598 ΔΔCt = 0.060 ± 0.242 | Sex × diet, 20.88 Sex, 19.90 Diet, 21.88 | Sex × diet, 0.0002*** Sex, 0.0003*** Diet, 0.0002*** |
Cx3cr1 | – | 2−ΔΔCt = 1.000 ΔΔCt = 0.000 ± 0.203 | 2−ΔΔCt = 0.1724 ΔΔCt = 2.520 ± 0.479 | 2−ΔΔCt = 1.147 ΔΔCt = − 0.200 ± 0.315 | 2−ΔΔCt = 1.207 ΔΔCt = − 0.270 ± 0.276 | Sex × diet, 16.44 Sex, 21.92 Diet, 14.71 | Sex × diet, 0.0007*** Sex, 0.0002*** Diet, 0.0011** |