Table 4 mHFD effects on microglial morphological parameters in the dorsal hippocampus CA1 stratum radiatum of PND30 offspring
Parameters | Mean ± standard error of the mean | F | p | |||
|---|---|---|---|---|---|---|
Male | Female | |||||
CD | mHFD | CD | mHFD | |||
Soma area (μm2) | 46.59 ± 1.92 | 46.88 ± 2.54 | 44.42 ± 1.38 | 45.82 ± 1.84 | Sex × diet, 0.08109 Sex, 0.6758 Diet, 0.1842 | Sex × diet, 0.7795 Sex, 0.4231 Diet, 0.6735 |
Arbor area (μm2) | 1352.68 ± 154.93 | 1355.63 ± 112.22 | 1374.23 ± 160.96 | 1377.16 ± 129.59 | Sex × diet, 4.378 × 10−9 Sex, 0.02340 Diet, 0.0004357 | Sex × diet, > 0.9999 Sex, 0.8803 Diet, 0.9836 |
Morphological index (a.u.) | 0.036 ± 0.003 | 0.035 ± 0.001 | 0.034 ± 0.003 | 0.035 ± 0.004 | Sex × diet, 0.09893 Sex, 0.1851 Diet, 0.0001109 | Sex × diet, 0.7572 Sex, 0.6728 Diet, 0.9917 |
Cell area (μm2) | 425.49 ± 86.24 | 430.71 ± 55.12 | 473.40 ± 79.36 | 435.13 ± 49.86 | Sex × diet, 0.09819 Sex, 0.1422 Diet, 0.05674 | Sex × diet, 0.7581 Sex, 0.7110 Diet, 0.8148 |
Circularity (a.u.) | 0.029 ± 0.001 | 0.027 ± 0.001 | 0.030 ± 0.001 | 0.027 ± 0.001 | Sex × diet, 0.03614 Sex, 0.7875 Diet, 4.683 | Sex × diet, 0.8516 Sex, 0.3880 Diet, 0.0459* |
Solidity (a.u.) | 0.281 ± 0.020 | 0.290 ± 0.018 | 0.302 ± 0.012 | 0.285 ± 0.006 | Sex × diet, 0.7345 Sex, 0.2907 Diet, 0.07122 | Sex × diet, 0.4041 Sex, 0.5972 Diet, 0.7930 |
Aspect ratio (a.u.) | 1.666 ± 0.055 | 1.648 ± 0.053 | 1.633 ± 0.065 | 1.663 ± 0.069 | Sex × diet, 0.1587 Sex, 0.02512 Diet, 0.008885 | Sex × diet, 0.6956 Sex, 0.8761 Diet, 0.9261 |
# Branches | 101.92 ± 21.70 | 104.47 ± 13.79 | 113.11 ± 17.14 | 102.23 ± 12.52 | Sex × diet, 0.1622 Sex, 0.07200 Diet, 0.06261 | Sex × diet, 0.6925 Sex, 0.7919 Diet, 0.8056 |
Average branch length (μm) | 3.318 ± 0.127 | 3.218 ± 0.075 | 3.172 ± 0.071 | 3.237 ± 0.041 | Sex × diet, 0.9630 Sex, 0.5690 Diet, 0.04635 | Sex × diet, 0.3410 Sex, 0.4616 Diet, 0.8323 |
Longest branch (μm) | 13.29 ± 0.24 | 13.26 ± 0.49 | 12.77 ± 0.21 | 13.99 ± 0.21 | Sex × diet, 4.048 Sex, 0.1145 Diet, 3.750 | Sex × diet, 0.0614 Sex, 0.7395 Diet, 0.0707 |
# Junctions | 52.38 ± 12.05 | 53.49 ± 7.28 | 58.43 ± 9.08 | 52.28 ± 6.78 | Sex × diet, 0.1616 Sex, 0.07164 Diet, 0.07779 | Sex × diet, 0.6930 Sex, 0.7924 Diet, 0.7839 |