Table 5 mHFD effects on microglial morphological parameters in the dorsal hippocampus CA1 stratum lacunosum moleculare of PND30 offspring
Parameters | Mean ± standard error of the mean | F | p | |||
|---|---|---|---|---|---|---|
Male | Female | |||||
CD | mHFD | CD | mHFD | |||
Soma area (μm2) | 40.18 ± 1.74 | 42.45 ± 2.06 | 40.14 ± 0.92 | 40.64 ± 1.57 | Sex × diet, 0.2961 Sex, 0.3230 Diet, 0.7283 | Sex × diet, 0.5938 Sex, 0.5777 Diet, 0.4060 |
Arbor area (μm2) | 988.76 ± 125.44 | 908.98 ± 111.46 | 974.04 ± 118.48 | 973.34 ± 157.09 | Sex × diet, 0.09350 Sex, 0.03685 Diet, 0.09685 | Sex × diet, 0.7637 Sex, 0.8502 Diet, 0.7597 |
Morphological index (a.u.) | 0.043 ± 0.004 | 0.049 ± 0.005 | 0.044 ± 0.006 | 0.047 ± 0.009 | Sex × diet, 0.04385 Sex, 7.575 × 10−5 Diet, 0.5447 | Sex × diet, 0.8368 Sex, 0.9932 Diet, 0.4712 |
Cell area (μm2) | 272.57 ± 46.35 | 272.50 ± 36.57 | 269.99 ± 40.25 | 283.11 ± 43.67 | Sex × diet, 0.02479 Sex, 0.009177 Diet, 0.02427 | Sex × diet, 0.8769 Sex, 0.9249 Diet, 0.8782 |
Circularity (a.u.) | 0.028 ± 0.003 | 0.033 ± 0.003 | 0.031 ± 0.003 | 0.032 ± 0.006 | Sex × diet, 0.2333 Sex, 0.1162 Diet, 0.6419 | Sex × diet, 0.6357 Sex, 0.7377 Diet, 0.4348 |
Solidity (a.u.) | 0.258 ± 0.012 | 0.287 ± 0.008 | 0.263 ± 0.007 | 0.282 ± 0.013 | Sex × diet, 0.2641 Sex, 0.004823 Diet, 5.616 | Sex × diet, 0.6144 Sex, 0.9455 Diet, 0.0307* |
Aspect ratio (a.u.) | 1.803 ± 0.089 | 1.787 ± 0.058 | 1.793 ± 0.078 | 1.752 ± 0.094 | Sex × diet, 0.02490 Sex, 0.07486 Diet, 0.1260 | Sex × diet, 0.8766 Sex, 0.7879 Diet, 0.7273 |
# Branches | 72.83 ± 12.86 | 73.24 ± 9.70 | 73.90 ± 11.91 | 77.18 ± 12.65 | Sex × diet, 0.01466 Sex, 0.04465 Diet, 0.02438 | Sex × diet, 0.9052 Sex, 0.8353 Diet, 0.8779 |
Average branch length (μm) | 3.464 ± 0.077 | 3.262 ± 0.048 | 3.420 ± 0.120 | 3.291 ± 0.037 | Sex × diet, 0.2168 Sex, 0.008423 Diet, 4.553 | Sex × diet, 0.6477 Sex, 0.9280 Diet, 0.0487* |
Longest branch (μm) | 13.61 ± 0.46 | 12.51 ± 0.55 | 12.96 ± 0.57 | 12.98 ± 0.61 | Sex × diet, 1.024 Sex, 0.02989 Diet, 0.9634 | Sex × diet, 0.3265 Sex, 0.8649 Diet, 0.3409 |
# Junctions | 36.88 ± 6.99 | 37.01 ± 5.19 | 37.61 ± 6.37 | 39.29 ± 6.71 | Sex × diet, 0.01468 Sex, 0.05614 Diet, 0.02034 | Sex × diet, 0.9051 Sex, 0.8157 Diet, 0.8884 |