Table 6 mHFD effects on microglial ultrastructure in the dorsal hippocampus CA1 stratum radiatum of PND30 offspring
Parameters | Mean ± standard error of the mean | F | p | |||||
|---|---|---|---|---|---|---|---|---|
Male | Female | |||||||
CD | mHFD | CD | mHFD | |||||
Organelles | # Lysosome | Primary | 1.405 ± 0.461 | 1.150 ± 0.317 | 0.878 ± 0.213 | 1.438 ± 0.258 | Sex × diet, 1.552 Sex, 0.1344 Diet, 0.2160 | Sex × diet, 0.2149 Sex, 0.7144 Diet, 0.6428 |
Secondary | 0.108 ± 0.052 | 0.200 ± 0.096 | 0.146 ± 0.075 | 0.188 ± 0.083 | Sex × diet, 0.1028 Sex, 0.02644 Diet, 0.7068 | Sex × diet, 0.7490 Sex, 0.8711 Diet, 0.4019 | ||
Tertiary | 0.027 ± 0.027 | 0.025 ± 0.025 | 0.000 ± 0.000 | 0.031 ± 0.031 | Sex × diet, 0.5148 Sex, 0.2007 Diet, 0.3970 | Sex × diet, 0.4742 Sex, 0.6548 Diet, 0.5296 | ||
# Lipofuscin | 0.135 ± 0.079 | 0.100 ± 0.048 | 0.073 ± 0.041 | 0.063 ± 0.043 | Sex × diet, 0.04895 Sex, 0.8091 Diet, 0.1716 | Sex × diet, 0.8252 Sex, 0.3699 Diet, 0.6793 | ||
# Endosome | Empty | 0.108 ± 0.052 | 0.200 ± 0.089 | 0.098 ± 0.058 | 0.063 ± 0.043 | Sex × diet, 0.9319 Sex, 1.267 Diet, 0.1867 | Sex × diet, 0.3360 Sex, 0.2621 Diet, 0.6663 | |
Content | 0.432 ± 0.120 | 0.250 ± 0.128 | 0.268 ± 0.086 | 0.188 ± 0.070 | Sex × diet, 0.2278 Sex, 1.132 Diet, 1.528 | Sex × diet, 0.6339 Sex, 0.2890 Diet, 0.2185 | ||
# Dilated ER/Golgi | 5.892 ± 0.945 | 6.000 ± 1.046 | 6.171 ± 1.069 | 5.188 ± 0.723 | Sex × diet, Sex, Diet, | Sex × diet, 0.5814 Sex, 0.7874 Diet, 0.6584 | ||
# Mitochondrion | Elongated | 0.459 ± 0.148 | 0.450 ± 0.138 | 0.293 ± 0.094 | 0.375 ± 0.087 | Sex × diet, 0.1409 Sex, 0.9777 Diet, 0.08878 | Sex × diet, 0.7080 Sex, 0.3244 Diet, 0.7662 | |
Total | 2.270 ± 0.365 | 3.075 ± 0.486 | 2.195 ± 0.309 | 2.906 ± 0.322 | Sex × diet, 0.01475 Sex, 0.1002 Diet, 3.870 | Sex × diet, 0.9035 Sex, 0.7521 Diet, 0.0511 | ||
Interactions with microenvironment | # Synaptic element | Pre-synaptic | 6.243 ± 0.504 | 9.075 ± 0.958 | 7.683 ± 0.808 | 7.688 ± 0.647 | Sex × diet, 3.521 Sex, 0.008597 Diet, 3.111 | Sex × diet, 0.0701 Sex, 0.9732 Diet, 0.0692 |
Post-synaptic | 3.081 ± 0.286 | 4.150 ± 0.438 | 3.707 ± 0.457 | 3.875 ± 0.559 | Sex × diet, 1.028 Sex, 0.1562 Diet, 1.936 | Sex × diet, 0.3123 Sex, 0.6933 Diet, 0.1663 | ||
# Myelinated axon | 0.135 ± 0.069 | 0.200 ± 0.089 | 0.073 ± .054 | 0.031 ± 0.031 | Sex × diet, 0.6218 Sex, 2.949 Diet, 0.02917 | Sex × diet, 0.4280 Sex, 0.0880 Diet, 0.8646 | ||
# Degenerating myelin | 0.216 ± 0.079 | 0.150 ± 0.067 | 0.098 ± 0.047 | 0.042 ± 0.042 | Sex × diet, 0.006245 Sex, 3.020 Diet, 0.8740 | Sex × diet, 0.9371 Sex, 0.0845 Diet, 0.3515 | ||
# Brain cell or vasculature | Astrocyte | 0.054 ± 0.038 | 0.225 ± 0.067 | 0.049 ± 0.034 | 0.031 ± 0.031 | Sex × diet, 4.104 Sex, 4.576 Diet, 2.719 | Sex × diet, 0.0446* Sex, 0.0341* Diet, 0.1013 | |
Neuron | 0.000 ± 0.000 | 0.125 ± 0.053 | 0.122 ± 0.052 | 0.094 ± 0.052 | Sex × diet, 2.771 Sex, 0.9714 Diet, 1.106 | Sex × diet, 0.0981 Sex, 0.3260 Diet, 0.2946 | ||
Oligodendrocyte | 0.000 ± 0.000 | 0.000 ± 0.000 | 0.000 ± 0.000 | 0.000 ± 0.000 | N/A | N/A | ||
Blood vessel | 0.189 ± 0.065 | 0.075 ± 0.042 | 0.073 ± 0.041 | 0.031 ± 0.031 | Sex × diet, 0.5805 Sex, 2.837 Diet, 2.709 | Sex × diet, 0.4473 Sex, 0.0942 Diet, 0.1019 | ||
# Extracellular space | 0.216 ± 0.079 | 0.650 ± 0.146 | 0.537 ± 0.168 | 0.500 ± 0.168 | Sex × diet, 2.596 Sex, 0.3406 Diet, 1.851 | Sex × diet, 0.1093 Sex, 0.5604 Diet, 0.1757 | ||
# Extracellular digestion | 0.135 ± 0.057 | 0.275 ± 0.101 | 0.366 ± 0.120 | 0.125 ± 0.059 | Sex × diet, 4.154 Sex, 0.1867 Diet, 0.2923 | Sex × diet, 0.0433* Sex, 0.6663 Diet, 0.5896 | ||