Table 7 mHFD effects on microglial ultrastructure in the dorsal hippocampus CA1 stratum lacunosum moleculare of PND30 offspring
Parameters | Mean ± standard error of the mean | F | p | |||||
|---|---|---|---|---|---|---|---|---|
Male | Female | |||||||
CD | mHFD | CD | mHFD | |||||
Organelles | # Lysosome | Primary | 1.556 ± 0.419 | 1.357 ± 0.258 | 1.229 ± 0.239 | 1.182 ± 0.202 | Sex × diet, 0.06292 Sex, 0.6902 Diet, 0.1644 | Sex × diet, 0.8023 Sex, 0.4076 Diet, 0.6858 |
Secondary | 0.361 ± 0.090 | 0.250 ± 0.098 | 0.229 ± ± 0.092 | 0.212 ± 0.104 | Sex × diet, 0.2389 Sex, 0.7742 Diet, 0.4337 | Sex × diet, 0.6259 Sex, 0.3806 Diet, 0.5113 | ||
Tertiary | 0.028 ± 0.028 | 0.000 ± 0.000 | 0.000 ± 0.000 | 0.030 ± 0.030 | Sex × diet, 1.817 Sex, 0.003435 Diet, 0.003435 | Sex × diet, 0.1800 Sex, 0.9534 Diet, 0.9534 | ||
# Lipofuscin | 0.111 ± 0.053 | 0.107 ± 0.060 | 0.029 ± 0.029 | 0.061 ± 0.042 | Sex × diet, 0.1493 Sex, 1.918 Diet, 0.09070 | Sex × diet, 0.6999 Sex, 0.1684 Diet, 0.7638 | ||
# Endosome | Empty | 0.194 ± 0.078 | 0.107 ± 0.079 | 0.229 ± 0.101 | 0.091 ± 0.051 | Sex × diet, 0.09748 Sex, 0.01231 Diet, 1.945 | Sex × diet, 0.7554 Sex, 0.9118 Diet, 0.1655 | |
Content | 0.361 ± 0.144 | 0.214 ± 0.079 | 0.171 ± 0.077 | 0.152 ± 0.063 | Sex × diet, 0.3989 Sex, 1.579 Diet, 0.6886 | Sex × diet, 0.5288 Sex, 0.2113 Diet, 0.4082 | ||
# Dilated ER/Golgi | 5.611 ± 0.788 | 7.643 ± ± 1.413 | 5.800 ± 0.805 | 7.512 ± 1.168 | Sex × diet, 0.1075 Sex, 0.02125 Diet, 2.659 | Sex × diet, 0.7436 Sex, 0.8843 Diet, 0.1054 | ||
# Mitochondrion | Elongated | 0.472 ± 0.180 | 0.500 ± 0.159 | 0.257 ± 0.118 | 0.485 ± 0.235 | Sex × diet, 0.3099 Sex, 0.4110 Diet, 0.5061 | Sex × diet, 0.5787 Sex, 0.5226 Diet, 0.4781 | |
Total | 3.111 ± 0.534 | 2.071 ± 0.430 | 2.400 ± 0.341 | 2.970 ± 0.543 | Sex × diet, 2.856 Sex, 0.03862 Diet, 0.2435 | Sex × diet, 0.0935 Sex, 0.8445 Diet, 0.6225 | ||
Interactions with microenvironment | # Synaptic element | Pre-synaptic | 6.250 ± 0.745 | 5.821 ± 0.587 | 5.171 ± 0.527 | 5.125 ± 0.442 | Sex × diet, 0.1168 Sex, 2.138 Diet, 0.1406 | Sex × diet, 0.7331 Sex, 0.1462 Diet, 0.7083 |
Post-synaptic | 3.306 ± 0.378 | 3.036 ± 0.369 | 3.629 ± 0.482 | 3.030 ± 0.300 | Sex × diet, 0.1726 Sex, 0.1614 Diet, 1.206 | Sex × diet, 0.6785 Sex, 0.6885 Diet, 0.2742 | ||
# Myelinated axon | 0.417 ± 0.108 | 0.536 ± 0.167 | 0.486 ± 0.161 | 0.545 ± 0.151 | Sex × diet, 0.04059 Sex, 0.07164 Diet, 0.3689 | Sex × diet, 0.8406 Sex, 0.7894 Diet, 0.5447 | ||
# Degenerating myelin | 0.083 ± 0.047 | 0.179 ± 0.090 | 0.200 ± 0.069 | 0.121 ± 0.058 | Sex × diet, 1.763 Sex, 0.2047 Diet, 0.01575 | Sex × diet, 0.1867 Sex, 0.6517 Diet, 0.9003 | ||
# Brain cell or vasculature | Astrocyte | 0.028 ± 0.028 | 0.214 ± 0.094 | 0.114 ± 0.055 | 0.061 ± 0.042 | Sex × diet, 4.604 Sex, 0.3601 Diet, 1.408 | Sex × diet, 0.0338* Sex, 0.5495 Diet, 0.2376 | |
Neuron | 0.056 ± 0.039 | 0.107 ± 0.060 | 0.000 ± 0.000 | 0.000 ± 0.000 | Sex × diet, 0.6095 Sex, 6.062 Diet, 0.6095 | Sex × diet, 0.4364 Sex, 0.0151* Diet, 0.4364 | ||
Oligodendrocyte | 0.000 ± 0.000 | 0.036 ± 0.036 | 0.000 ± 0.000 | 0.000 ± 0.000 | Sex × diet, 1.384 Sex, 1.384 Diet, 1.384 | Sex × diet, 0.2417 Sex, 0.2417 Diet, 0.2417 | ||
Blood vessel | 0.056 ± 0.039 | 0.143 ± 0.067 | 0.114 ± 0.055 | 0.152 ± 0.063 | Sex × diet, 0.2002 Sex, 0.3625 Diet, 1.238 | Sex × diet, 0.6553 Sex, 0.5482 Diet, 0.2679 | ||
# Extracellular space | 0.556 ± 0.176 | 0.214 ± 0.094 | 0.514 ± 0.180 | 0.061 ± 0.042 | Sex × diet, 0.1533 Sex, 0.4611 Diet, 7.666 | Sex × diet, 0.6961 Sex, 0.4984 Diet, 0.0065** | ||
# Extracellular digestion | 0.528 ± 0.216 | 0.321 ± 0.219 | 0.200 ± 0.090 | 0.364 ± 0.105 | Sex × diet, 1.231 Sex, 0.7334 Diet, 0.01641 | Sex × diet, 0.2693 Sex, 0.3934 Diet, 0.8983 | ||