Fig. 1

Infiltration of peripheral leukocytes, including monocytes and neutrophils, are reduced in Cx3cr1-Cre::Panx1^fl/fl mice compared to Panx1^fl/fl mice. Representative flow cytometry plots for (a) peripheral leukocytes and microglia, (b) inflammatory monocytes, and (c) neutrophils 3-days post-injury. Quantification indicates that the number of (d) CD45^hi CD11b⁺ peripheral leukocytes (**P < 0.01, ***P < 0.001, one-way ANOVA (F_3,23 = 13.31), Tukey’s post hoc test), (f) CD45^hi and Ly6c inflammatory monocytes (**P < 0.01, ***P < 0.001, one-way ANOVA (F_3,12 = 19.62), Tukey’s post hoc test) and (g) neutrophils (**P < 0.01, ***P < 0.001, one-way ANOVA (F_3,12 = 27.31), Tukey’s post hoc test) were reduced in Cx3cr1-Cre::Panx1^fl/fl mice compared to Panx1^fl/fl mice after TBI. (e) shows that the number of microglia are not changed between the genotypes. Values are expressed as mean (±SD) mean fluorescence intensity relative to the background. n = 4 to 12 mice per group