Fig. 4

Protein expression pattern of TNF-α and IL-10 after the Hb and RvD1 treatment. a, c Time course of protein expression after Hb stimulation. Microglia were cultured in 20 μM Hb for 1 to 24 h (n = 3). b, d Effect of RvD1 on Hb-induced TNF-α and IL-10 expression at different time points. Microglia were cultured in medium containing 25 nM RvD1 for 30 min, and then cultured in 20 μM Hb for 1–24 h (n = 3). The data were analyzed by two-way ANOVA and Sidak post hoc multiple comparison. **p < 0.01. n is the number of independent cell samples