Fig. 5

Effects of RvD1on the IRAK1/TRAF6/NF-κB signaling pathway. a, d The nuclear translocation of p65. Microglia were cultured in medium containing 25 nM RvD1 for 30 min, which was followed by 15 min of Hb stimulation (n = 3). b, c mRNA expression changes in IRAK1 and TRAF6 levels after RvD1 treatment. Microglia were cultured in medium containing 25 nM RvD1 for 30 min before being cultured in 20 μM Hb for 1–24 h (n = 3). Data were analyzed by one-way ANOVA followed by Tukey’s post hoc multiple comparison for a, d and two-way ANOVA followed by Sidak post hoc multiple comparison for b, c. *p < 0.05, ***p < 0.001, and ##p < 0.01. Bar = 40 μm. n is the number of independent cell samples