Fig. 8

Influence of RvD1 on neuronal death induced by Hb. The primary neurons were cultured in a medium containing 50 μM Hb for 24 h, and RvD1 was added at a concentration of 25 nM 30 min before Hb stimulation. a, c Live-dead cell staining and the corresponding quantitative statistical results (n = 3). The red color shows PI staining, indicating dead cells; the green color shows calcein AM staining, indicating live cells. b, d The western blot results of Bax, bcl-xL, cleaved caspase-3, caspase-3, and the corresponding semi quantitative statistical results (n = 3). The data were analyzed by one-way ANOVA and Tukey’s post hoc multiple comparison. *p < 0.05, ***p < 0.001, #p < 0.05, ##p < 0.01 and ns showed no significant difference. n is the number of independent cell samples. Bar = 50 μm