Fig. 8

MiR-195 prevented the microglial/macrophage polarization to M1 phenotype induced by 2VO surgery. a MiR-195 expression in the hippocampus of 2VO rats at 8 weeks with or without lenti-pre-miR-195 treatment was detected by qRT-PCR. Bars represent the mean ± SD. n = 6. *P < 0.05 vs sham group, ^#P < 0.05 vs 2VO. b MiR-195 decreased the percentage of CD68 in the Iba-1⁺ cells in hippocampal DG and CA1 region of 2VO rats. c MiR-195 did not affect the percentage of CD206 in the Iba-1⁺ cells either in hippocampal DG region or CA1 region of 2VO rats. d MiR-195 reversed the increased ratio of CD68/CD206 in the hippocampal DG and CA1 regions of 2VO rats. Bars represent the mean ± SD, n = 9 slices from 3 animals per group. *P < 0.05 vs sham group, ^#P < 0.05 vs 2VO. e, f Representative images of CD68 and CD206 expression in the Iba-1⁺ cells of hippocampal DG (e) and CA1 (f) region of 2VO rats following the stereotaxic injection of lenti-pre-miR-195 into CA1 region. The scale bar was 40 μm. g Lenti-pre-miR-195 injection reversed the increased mRNA level of TNF-α in the hippocampus of 2VO rats. h Lenti-pre-miR-195 injection reversed the increased mRNA level of IL-1β in the hippocampus of 2VO rats. i Lenti-pre-miR-195 injection did not affect TGF-β level in the hippocampus of 2VO rats. j Lenti-pre-miR-195 injection inhibited the increased CX3CL1 and CX3CR1 in the hippocampus of 2VO rats. Bars represent the mean ± SD, n = 6. *P < 0.05 vs sham group, ^#P < 0.05 vs 2VO. All data were analyzed using one-way ANOVA followed by Tukey test