Fig. 4
Effects of rhFGF21 on migrated peripheral immune cell infiltration in the CNS. a Representative flow cytometry analysis shows the gating strategy for NK cells (CD45highCD3-NK1.1+), CD4+T cells (CD45highCD3+CD4+), CD8+T cells (CD45high CD3+CD8+), macrophages (CD11b+CD45highF4/80+), neutrophils (CD11b+CD45highLy-6G+), and monocyte cells (CD11b+CD45highLy-6G−Ly-6C+) using FMO. b Gating strategy of CD68, CD86, and CD206 from the subsets of macrophages using FMO control. c Quantification analysis shows the cumulative data for migrated peripheral immune cells at 3 days post-stroke. d Protein levels of CD68, CD86, and CD206 expressed in macrophages. e, f Line graphs summarize the flow cytometry data showing the dynamic distribution of infiltrated immune cells defined as CD45high (e) and macrophages (f) in the brain at 1, 3, and 7 days after stroke. g–i Temporal presence of CD68+ (g), CD86+ (h), and CD206+ (i) macrophages at 1, 3, and 7 days after stroke among the three groups. n = 6 in the sham group, n = 12 in the MCAO and rhFGF21-treated group. Values are mean ± SEM by one-way ANOVA. p value (red) of MCAO versus sham, p value (green) of MCAO versus MCAO+rhFGF21