Fig. 7

Effects of FGF21 on inflammatory cytokines (IL-1β, TNF-α, IL-6, and TGF-β) in sorted microglia and macrophages. a Microglia (CD11b⁺CD45^int) from the brain, and macrophages (CD11b⁺CD45^highF4/80⁺) from the brain or spleen sorted by FACS coupled with MACS, producing a purity of above 90%. b–d qRT-PCR shows the gene expression of IL-1β, TNF-α, IL-6, and TGF-β in microglia (b) and macrophages from the brain (c) and macrophages from the spleen (d). n = 8 per group. Values are mean ± SEM by one-way ANOVA