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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: Anti-inflammatory IL-10 administration rescues depression-associated learning and memory deficits in mice

Fig. 7

STAT3 knockdown impairs novel object recognition in NLH mice and blocked IL-10 effects IL-10 to reverse impairments. Male mice were treated intranasally with a scrambled siRNA control or STAT3 siRNA (10 μg/mouse) 1 day prior to the induction of the learned helplessness paradigm and treated every day thereafter. Mice were subjected or not (NS) to the learned helplessness paradigm and separated into 2 groups: non-learned helpless (NLH) and learned helpless (LH) mice, according to their number of failures out of 30 escapable shock trials, and the next day, novel object recognition was assessed. After the cognitive assessments, mice were treated with IL-10 (5 μg/mouse) i.n. and retreated 1 h before the second novel object recognition re-assessment on the next day as shown in (a). The percentage of a mouse’s total object exploration time spent exploring the familiar (F) and novel (N) objects in scrambled siRNA and STAT3 siRNA treated mice and in IL-10-treated, scrambled siRNA- and STAT3 siRNA-treated mice (b) as well as the total time (s) mice spent engaged in exploration of both the familiar and novel objects combined (c) in the novel object recognition test were reported. Each dot represents a mouse. Two-way ANOVA F(14,68)interaction = 10.42, F(7,68)treatment = 24.11, F(2,68)condition = 3.088 × 10−13, Bonferroni post hoc test, *p < 0.05, compared to % time spent with familiar object, bars represent means ± SEM, n = 3–5 mice/group

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