Fig. 1

Experimental design. Pregnant dams were exposed to MIA with either LPS (2 mg/kg in 1 ml) beginning at GD7 and continuing every second day until delivery (a) or a single injection of Poly I:C (4 mg/kg in 1 ml) at GD15 (b). Control groups were subjected to vehicle (saline) injections in a corresponding manner. At PND7, some male offspring were sacrificed and used for biochemical and immunohistofluorescent analyses. The rest of the animals underwent the behavioural examination, including the PPI test at PND30 and PND90, the light-dark box test at PND88 and the social interaction test at PN92. Twenty-four hours after the last behavioural test (PND93), the rats were sacrificed by decapitation and the tissues were collected for biochemical examination