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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Serum amyloid A inhibits astrocyte migration via activating p38 MAPK

Fig. 2

Saa3 deficiency increases the number of activated astrocytes around Aβ plaques in the brain of APP/PS1 mice. Representative Western blots showing the GFAP expression in the cortex (a) and hippocampus (b) of 12-month-old WT, APP/PS1, Saa3/, and APP/PS1-Saa3/ mice. Quantification of the immunoreactivity of the blots normalized against GAPDH was shown in c and d. Results are expressed as the mean ± SEM, with 3–8 mice in each group. *p < 0.05; ***p < 0.001 compared with WT mice. #p < 0.05 compared with APP/PS1 mice. e Serial sections of 12-month-old WT, APP/PS1, Saa3/, and APP/PS1-Saa3/ mouse brain were stained for Aβ deposits using a rabbit anti-Aβ polyclonal antibody and Alexa Fluor 488-conjugated anti-rabbit IgG (green fluorescence). The sections were subsequently stained for GFAP using a monoclonal anti-GFAP Cy3TM antibody (red fluorescence). The cell nuclei were stained with DAPI (blue). The scale bar in the upper left panel is 300 μm. Selected areas are enlarged by seven times and shown as combined as well as individual fluorescence stains. Quantification of the GFAP fluorescence (f) and the number of astrocytes around Aβ deposits (g) was shown. Results are expressed as the mean ± SEM based on four individual fields for each region, using 2–3 mice in each group. *p < 0.05; ***p < 0.001 compared with WT mice. ##p < 0.01; ###p < 0.001 compared with APP/PS1 mice

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