Fig. 5
From: Serum amyloid A inhibits astrocyte migration via activating p38 MAPK
SAA inhibits actin and microtubule reorganization in astrocytes with scratch-wound assay. Primary cultures of astrocytes and U251 cells were treated with SAA (1 μM) or 10% FBS for scratch-wound assay. At 16 h after scratching, the cells were stained with anti-FITC-phalloidin (F-actin, green fluorescence) (a) or anti-α-tubulin (microtubule) polyclonal antibody and Alexa Fluor 488-conjugated anti-rabbit IgG (green fluorescence) (b). The cell nuclei were stained with DAPI (blue). Scale bar, 25 μm. The white dashed lines indicate the direction of the wound. Selected areas are enlarged by four times. For microtubule staining, primary astrocytes (c) and U251 cells (d) were scored as protruding when their protrusions were at least 4 times longer than wide. Results are expressed as the mean ± SEM based on three independent experiments, each in triplicate. *p < 0.05; **p < 0.01; ***p < 0.001 compared with the control (DMEM)