Fig. 4

BaP exposure induced neuronal loss only in APP/PS1 mice. a Representative images of immunohistochemical staining of NeuN in the cortex and the hippocampus. The quantification results of NeuN immunoreactivity are expressed as a percentage of the corresponding WT-Vehicle control. b Levels of NeuN protein in the cortex and the hippocampus were detected by Western blot. β-actin was used to monitor loading errors. Data are expressed as a percentage of the WT-Vehicle control. Data are mean ± SEM of 3–4 mice in each group. Significance was determined by two-way ANOVA followed by LSD multiple comparisons post-hoc test. **P < 0.01 compared with WT-Vehicle control, ^#P < 0.05 compared with WT-BaP group