Fig. 4
miR-155 inhibitor reduces infection-induced weight loss without altering bacterial load in liver and spleen. Male and female age-matched C57BL/6J mice were infected with 1.8–2.4 × 105 CFU Lm EGD and treated with antibiotics beginning 2d p.i. miR-155 inhibitor or LNA scramble was injected s.c. on 2d, 4d, and 6d p.i. a Animals were weighed daily. Weights are expressed as a percentage of initial pre-infection weight for each mouse individually. n = 8–18 animals for each time point and treatment. Significance was determined via two-way ANOVA. b–d Organs were removed aseptically at necropsy, then weighed and homogenized in sterile dH2O. Bacterial loads in spleen (b), liver (c), and brain (d) at 3d p.i. were measured by serial dilution and plating on agar. Significance was determined via two-tailed t test