Fig. 2

IL-33 ameliorates HI-induced brain injury in neonatal mice. a Representative photographs of TTC-stained coronal brain sections from IL-33- and PBS-treated mice at 7 days post HI (left). Right, infarct volumes at 7 days post HI (n = 12 per group). b Representative western blot images of cleaved caspase-3 (C-casp-3), Bax, and Bcl-2 from IL-33- and PBS-treated mice at 7 days post HI (n = 9 per group). c Densitometric analysis of data is shown in b. d Representative images of Neun (red) and TUNEL (green) in the cerebral cortex of IL-33- and PBS-treated mice at 7 days post HI (left). Scale bar, 25 μm. Right, quantification of Neun and TUNEL dual-labeled cells at 7 days post HI (n = 9 per group). e–g Neurobehavioral outcomes of the geotaxis reflex (left), cliff avoidance reaction (middle), and grip test (right) at 1, 3, and 7 days after HI (n = 9 per group). Data are mean ± SEM. *P <0.05 compared to untreated controls