Fig. 5

IL-33 promotes astrocyte survival and proliferation after HI injury. a Representative FACS analysis of ST2⁺ astrocytes at 24 h after 6 h OGD. Right, numbers indicate the percentage of ST2⁺ astrocytes at 24 h after 6 h OGD. Data are mean ± SEM (n = 3 in each group). *P < 0.05 compared to untreated controls. b CCK-8 assay in astrocyte-enriched cultures subjected to 6 h OGD or sham conditions followed by treatment with PBS or a range of concentrations of IL-33 for another 24 h. Data are mean ± SEM (n = 3 in each group). ^#P < 0.01 compared to untreated controls. c Representative images of Ki-67 (red) and GFAP (green) in the cerebral cortex of IL-33- and PBS-treated mice at 7 days post HI (left). Scale bar, 25 μm. Right, quantification of Ki-67 and GFAP dual-labeled cells at 7 days post HI (n = 9 per group). d, e Percentages of phosphorylated AKT (pAKT) (d) and Ki-67⁺ (e) in astrocytes at 24 h after 6 h OGD and the culture conditions shown. Data are mean ± SEM (n = 3 in each group). *P < 0.05, ^#P < 0.01. f Representative FACS analysis of cell-cycle status of astrocytes at 24 h after 6 h OGD and the culture conditions shown. Data are mean ± SEM (n = 3 in each group). *P < 0.05