Fig. 6

IL-33 protects astrocytes from apoptosis after OGD insult. a The percentage of annexin⁺ astrocytes at 24 h of culture with or without IL-33 and after 6 h OGD and the culture conditions shown. Data are mean ± SEM (n = 3 in each group). *P < 0.05 compared to untreated controls. b The percentage of TUNEL⁺ astrocytes from IL-33- and PBS-treated mice at 7 days post HI. Data are mean ± SEM (n = 6 in each group). *P < 0.05 compared to untreated controls. c Left, P53 mRNA expression in astrocytes at 12 h of culture with or without IL-33 and after 6 h OGD and the culture conditions shown. Right, mean percentages of P53 protein expression in astrocytes at 24 h of culture with or without IL-33 and after 6 h OGD and the culture conditions shown. Data are mean ± SEM (n = 3 in each group). *P < 0.05 compared to untreated controls. d The percentage of annexin⁺ astrocytes with or without P53 inhibition at 24 h of culture with or without IL-33 and after 6 h OGD and the culture conditions shown. Data are mean ± SEM (n = 3 in each group). *P < 0.05 compared to untreated controls. n.s. not significant. e Left, PUMA mRNA expression in astrocytes at 12 h of culture with or without IL-33 and after 6 h OGD and the culture conditions shown. Right, mean percentages of PUMA protein expression in astrocytes at 24 h of culture with or without IL-33 and after 6 h OGD and the culture conditions shown. Data are mean ± SEM (n = 3 in each group). *P < 0.05 compared to untreated controls. n.s. not significant. f The percentage of annexin⁺ astrocytes with or without PUMA knockdown at 24 h of culture with or without IL-33 and after 6 h OGD and the culture conditions shown. Data are mean ± SEM (n = 3 in each group). *P < 0.05 compared to untreated controls. n.s. not significant. g Ki-67 assay in astrocytes with or without PUMA knockdown at 24 h of culture with or without IL-33 and after 6 h OGD and the culture conditions shown. Data are mean ± SEM (n = 3 in each group). *P <0.05 compared to untreated controls. n.s. not significant