Fig. 4

CA-induced inflammatory cytokine expression in the brain was attenuated in MCC950-treated mice. Mice were subjected to sham or CA/CPR surgery. After 15-min reperfusion, mice received intraperitoneal injection of MCC950 (treated) or vehicle (control), followed by daily injection for 2 days. On day 3 post-CA, brains were collected for qRT-PCR and flow cytometry analyses. a Expression of cytokines. The mRNA levels (n = 4/group) of IL-1β, TNF-α, TGF-β, and IL-10 were measured by qRT-PCR. b Leukocyte infiltration into the post-CA brain. Single-cell suspensions were prepared from whole brains and subjected to flow cytometric analysis (n = 5/group). Infiltrating leukocytes (CD45^+hi) and CD45^+hiCD11b⁺ cells with their subsets, monocytes/macrophages (CD45^+hiCD11b⁺F4/80⁺), and neutrophils (CD45^+hiCD11b⁺Ly6G⁺) are quantified and the representative dot plots are also shown here. Data are presented as mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ns not significant